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The resulting cell lysate was filtered through a 0.2-m filter and loaded onto a 5-mL HisTrap HP column (GE Healthcare/Cytiva) equilibrated in wash buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM TCEP, 25 mM imidazole)

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To improve gastrointestinal absorption, a carrier molecule of sodium N-(8-[2-hydroxybenzoyl] amino) caprylate (SNAC) was added to its chemical structure to overcome the challenges of peptide degradation and poor membrane permeability [23]