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Sinet PM, Lejeune J, Jerome H

Biotin supplement: 2,500-5,000mcg daily

Protein concentrations were quantified using a BCA protein quantification kit according to the manufacturers protocol (Thermo Fisher Scientific, Rockford, U.S.A.), and equal amounts of proteins (~30 g) were resolved on 10%12.5% SDS-PAGE gels

The transgenic overexpression of HGF in the nervous system attenuates spinal motoneuronal death and axonal degeneration, delays onset of the disease, and prolongs the lifespan of SOD1-G93A mice (Sun et al., 2002)