Key evidence: A pilot RCT by Kumar et al
PlexusDx genetic testing may help provide context for individual variation in peptide pathway function

Abbreviations GLP-1 glucagon-like peptide 1 GEO gene expression omnibus GO gene ontology KEGG Kyoto encyclopedia of genes and genomes pathway PPI protein-protein interaction DEGs differentially expressed genes NTS nucleus tractus solitarius Bgn biglycan Mapkapk3 mitogen-activated protein kinase activated protein kinase 3 TCM traditional Chinese medicine CCK cholecystokinin GLP1-RA GLP-1 receptor agonist RIP-Seq RNA co-immunoprecipitation combined with high-throughput sequencing DVC dorsal vagal complex MBH medial basal hypothalamus logFC log value of gene fold change BP biological process MF molecular function CC cellular component FDR false discovery rate MCC maximal clique centrality rAAV adeno-associated virus cre cyclization recombination enzyme CNO clozapine-N-oxide WB Western blotting RT-qPCR reverse transcription-quantitative polymerase chain reaction cDNA complementary DNA IF immunofluorescence IOD integrated optical density AP posterior area ECM extracellular matrix Erk1/2 extracellular signal-regulated kinase JNK c-JunN-terminal kinase

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The gene expression of carnitine-acylcarnitine translocase (SLC25A20) in PBMC remained stable in CAR, whereas it was upregulated in CON during the LPS challenge, suggesting an adaptation to increased energy demands in CON