Subsequently, the bariatric surgeon will connect the gastric pouch to a specific part of the small intestine, so that the food passes directly into it
Flow cytometry of tumor infiltrating immune cells Mice were euthanized, and tumors were mechanically dissociated and digested in a mixture of enzymes (0.1% collagenase type IV, 0.01% hyaluronidase, 1% FBS, 20 U/mL DNase I dissolved in HBSS buffer) for 30 minutes at 37 C
PLoS One 9 , e88297 (2014)
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