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Preparation of protein lysates and Western blot For BAT samples, a piece of tissue was lysed in lysis buffer (50 mM HEPES pH 7.5, 1% Triton X-100, 50 mM tetrabasic sodium pyrophosphate, 100 mM sodium fluoride, 10 mM EDTA, 10 mM sodium o-vanadate, 1 mM phenylmethanesulfonyl fluoride and 10 g/mL protease inhibitors pH 7.47.6, using a tissue homogenizer (0003737000

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Smad3 is an important mediator of renal fibrosis (Meng et al., 2013)