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It is one of the most studied peptides in cellular signalling research, referenced extensively for its interactions with collagen synthesis pathways, antioxidant gene expression, and tissue remodelling mechanisms
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To control for intermolecular effects, a natural abundance sample of the MTSL-peptide was mixed in a 1:1 ratio with a 15 N-labeled Cys1253Ser/Cys1254Ser mutant S tail peptide, and peak intensities were measured for the oxidized and reduced states as above