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PCR was performed in 20 l reaction mixture containing 5 ng of the obtained cDNA (or 50 ng of human genomic DNA as a control), 1x Phusion HF buffer, 0.2 mM dNTP mix, 0.5 M of each primer and 0.4 U Phusion DNA polymerase (F530S, Thermo Scientific)

After complete drying, it was fixed using 2% phosphotungstic acid for 10 min

Therapeutic angiogenesis using HGF plasmid

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When formulated as a 150 mg sublingual troche, glutathione is designed to be absorbed directly through the oral mucosa, which may provide a rapid and efficient route of administration, bypassing the gastrointestinal tract and first-pass metabolism in the liver