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Real-time PCR experiments have been performed according to MIQE guidelines 57 in CFX96 Real-time systems (BioRad)

J Cell Biochem

Food Res Int 64:248255

Review of molecular mechanisms involved in the activation of the Nrf2-ARE signaling pathway by chemopreventive agents

The 1st glutathione-interaction site, also termed glutathione-scaffold site, is rather well defined and comprises several protein areas that altogether contribute to catalysis by interacting with the glutathione moiety of GSSR 7,8,13,14,15,35 , therefore explaining the specificity for the -glutamyl moiety 9,49,50

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