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The cells were subsequently received different treatments for 24 h and were cultured in a serum-free medium containing a labeling media, which consisted of BODIPY cholesterol (0.025 mM) (BODIPY-Cholesterol, Avanti Polar Lipids, Alabaster, AL, United States), MCD (10 mM, sigma), HEPES (Sigma), and egg phosphatidylcholine (0.1 mM) (Avanti Polar Lipids) ( g for 5 min the lysate supernatant was collected or fluorescence intensity determination

After washing, internalized agonist fluorescence was measured over 60 min

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This medication may cause fetal harm

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