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Similarly, several nonalcoholic fatty liver diseases (NAFLDs), IBDs, hepatocellular carcinoma, cardiovascular diseases (CVDs), alcoholic liver disease (ALD), chronic kidney diseases (CKDs), and cirrhosis are associated with the gut microbiota and its metabolites (Toumi et al., 2021)

The resulting reaction mixture was centrifuged at 15,000 g for 10 min, and the supernatant was analyzed by LC-FL (Waters, Milford, MA, USA) and LC-MS/MS (Shimazu, Kyoto, Japan)