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The gap between effective and ineffective use comes down to understanding that the dihexa half life dictates dosing frequency, but therapeutic outcomes are determined by what happens at the synaptic level during those brief exposure windows
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Common side effects Injection-site reactions: mild redness, tenderness, swelling, occasional bruising - usually resolve within hours Skin flushing / warmth - brief, related to vasodilation Transient fatigue in the first 12 weeks of intensive protocols Mild headache - usually dose-related Higher-dose theoretical concerns Copper accumulation - each 2 mg injection adds roughly 0.03 mg of elemental copper, trivial next to the 0.92.2 mg from a normal diet
Other researchers have pointed out that even pharmaceutical-grade methylene blue contains impurities, and that industrial-grade or chemical-grade methylene blue, like that sold as a stain or dye, should not be used in humans or animals
10.1007/s00213-015-4095-7 49 KumazawaY.ItagakiA.FukumotoM.FujisawaH.NishimuraC.NomotoK