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An IV drip delivers fluids and selected nutrients directly through an IV and usually takes longer

Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems

Sigma) incorporated in the gels was photoactivated on a ChemiDoc (BioRad) using the stain-free gel application (590/110 filter and 45 s of activation)

Not in a controlled setting

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