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SLC7A11 overexpression inhibits pyroptosis by ensuring robust GSH levels and reduced ROS 62 , whereas inhibition of glutamine uptake causes decreased GSH and increased ROS, leading to GSDME-dependent pyroptosis in non-small cell lung cancer (NSCLC) cells 63

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The ICS was performed as previously described ( Supplementary Table 1 ) and then fixed and permeabilized using a Cytofix/Cytoperm kit (BD Bioscience) or a Transcription Factor Buffer Set (BD Biosciences) and stained with the corresponding antibodies (IFN-, IL-21, IL-10, FoxP3, TCF1, and Eomes)

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