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Saliva test - Cotinine may be detected for 7 to 14 days

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The cells were long term exposed to CSE 10% and 20% diluted in serum-free medium (MEM or RPMI1640), for 2 hours per day, for 7, and 14 days to simulate the chronic treatment with cigarette smoke as previously described with some modifications 26

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1 2 3 "Wake Forest Debates Use of RJR Gift," The Charlotte Observer , February 7, 1987

Regional Preferences and Habits In some countries, smoking remains deeply ingrained in the culture, while in others, smoking rates are steadily declining