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As COL IV genes are expressed by the animals body wall muscles in addition to their DTCs 44 , to avoid any confounding effects that could result from systemic manipulations of COL IV levels, we used a strain that allows us to specifically interfere with DTC COL IV production by RNAi
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Spatial transcriptomics Tissue preparation and staining Fresh frozen brains from 8-week-old male chow-fed SpragueDawley rats (Janvier, France) were sectioned at a thickness of 12 m to represent coronal and sagittal cuts of the DVC
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