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For the TNBS-induced colitis model, approximately 5 10 6 Th17 cells from cigarette-exposed Gpr15 +/+ and Gpr15 / mice were transferred into Gpr15 / mice via tail vein injection on the third day of colitis induction, and the clinical signs of colitis were monitored

Subsequently, cells were incubated in 10 g/mL anti-iNOS or 20 g/mL anti-eNOS (BD Transduction Laboratories) for 30 mins on ice, washed twice and added goat anti-mouse IgG conjugated with FITC (DAKO) for further 30 mins in the dark on ice

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