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In this review, we explore the complex interplay between sleep, metabolism, and aging across five core themes: (1) the role of sleep in maintaining optimal free radical levels, (2) whether ROS directly contribute to or mediate sleep disturbances in aging, (3) how age-related sleep disturbances may, in turn, contribute to impaired brain metabolism and exacerbate age-related changes, (4) the role of sleep, redox, and aging in peripheral systems, and (5) the evidence that improving mitochondrial redox potential restores sleep and slows aging, as well as the potential benefits of interventions like exercise, antioxidant-rich foods, supplements, and specialized diets that enhance sleep and/or metabolic efficiency, and their effects on aging markers (summarized in Figure 1)

Potential Side Effects High doses can produce adverse reactions such as insomnia, anxiety, or fatigue if escalated too quickly

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The assembled RAGRagulator complex was eluted using wash buffer with 250 mM imidazole and further purified by SEC with a Superdex 200 10/300 GL column

The study was approved by the Ethics Committee of the Second Affiliated Hospital of Guangzhou Medical University

While the RDA is 2.6 mcg, studies show that higher doses of up to 1,000 mcg (1 mg) daily are generally considered safe during pregnancy, especially when treating deficiency [*]