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The sites can be formed when certain relatively unstable DNA adducts, such as 7-methyldeoxyguanosine, lose their purine base (in this case 7-methylguanine) due to spontaneous decomposition or hydrolysis

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For this reason, filler is usually a blend of different leaves to achieve a mixture of taste and burning qualities

Briefly, human in vitro systems do not reflect all aspects of cellular interaction and tissue organization, in vivo animal models do not necessarily reflect human biology in its finer details, and human clinical studies are hampered by time and cost constraints as well as accessibility to key biological samples (that are accessible in animal studies)

The DCs subsets have mouse counterparts as well [117]

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