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PMID 15386533

The other non-perfused brain regions were snap frozen in 80 C 2-methylbutane and stored in a 80 C freezer until further processing for protein extraction

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These findings are mechanistically coherent with GHK-Cus anti-inflammatory NF-kB activity because the secondary injury cascade in SCI involves microglial activation, sustained pro-inflammatory cytokine release, and reactive oxygen species generation