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However, their fragility and neutral thermal conductivity make them prone to breaking and less effective at cooling smoke

Much of their tobacco packaging used the castle trademark, providing an inextricable link between the brand and Nottingham no matter where you were in the world

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This resulted in the movement of many Ottoman tobacco merchants, usually ethnic Greeks, to Egypt, a country which was culturally similar to and was arguably de jure a part of the Ottoman Empire but outside the tobacco monopoly as a result of its de facto occupation by the United Kingdom

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The coverslips were flipped over onto ProLong diamond antifade mountant with DAPI (Invitrogen, CA, USA)-loaded slide glasses and incubated overnight at 4 C