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The PBMCs were aliquoted (50 l/well) into 96-well flat-bottom tissue culture plates pre-coated or not as above with 0.5 g/mL of anti-human CD3 and then 2 g/mL anti-human CD28 added

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The Beatles second cinematic foray 1965s Help!, which began filming 55 years ago today has long lingered in the shadow of the previous years screen debut, A Hard Days Night