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Readouts most often include glycerol release and free fatty acid quantification in the supernatant, alongside cellular ATP or viability measures to confirm that observed lipolysis reflects genuine metabolic signaling rather than cytotoxic membrane disruption

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doi: 10.1111/j.1468-0009.2011.00648.x 124 MacFarlaneECareyRKeegelTEl-ZaemaySFritschiL

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J Controll Rel 301:176189 Zelba H, McQueeney A, Rabsteyn A, Bartsch O, Kyzirakos C, Kayser S, Harter J, Latzer P, Hadaschik D et al (2022) Adjuvant treatment for breast cancer patients using individualized neoantigen peptide vaccinationa retrospective observation