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Quantitative PCR To analyze mRNA expression, total RNA was collected from cells using the PureLink RNA Mini Kit (Thermo Fisher Scientific), and 1 g was reverse-transcribed using the ProtoScript II First Strand cDNA Synthesis Kit with oligo(dT) primers and murine RNase inhibitor (New England Biolabs)

The incidence and speed of DR progression correlate with age in diabetic patients

Avoid contact with reducing agents , cysteine, glutathione, ascorbate, DTT

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Copper Peptide vs the Field The three peptides are complementary rather than competing