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At the end of treatment, mice were euthanized by asphyxiation with CO 2 and peripheral blood, spleen single cell population were analyzed for the ROS, ferroptosis levels by DCF and Bodipy-C11 staining followed by flow cytometry analysis

Cost and availability: Allulose is currently more expensive than traditional sugars and many other sweeteners, which may limit its widespread use

Control rats (Group 1) received 0.1 ml of saline throughout the experiment

Patients that had received selective serotonin reuptake inhibitors (SSRIs) within the past 6 months were excluded, since SSRIs may alter S-5-HT levels (35), resulting in exclusion of 7 hypothyroidism and 2 CFS patients

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