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495 The GOF properties of mutant p53 involve more than just destabilizing the p53 protein

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Results Validation Success: Method validated per ICH M10 guidelines for 1500 ng/mL range Stability: Demonstrated 64-day stability at both 20C and 80C Selectivity: Chromatograms free of interferences across all plasma types Matrix Effects: Assay free of matrix effects across all plasma types

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Heated the reaction mixture to 50-55C and stirred for 45 min

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