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[14] and Kenkel et al

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The nicotine effect on the adrenal gland can lead to an elevation in testosterone, but also increases levels of adrenaline (causing rises in blood pressure), and cortisol (a stress hormone)

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Mitochondrial ROS production was assessed by staining the cells with 5 M of MitoSOX (Molecular Probes/Invitrogen, Carlsbad, CA, USA) for 30 min at 37 C