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Briefly, metabolites in plasma (100 L) were extracted with 90% methanol (900 L), and proteins were precipitated at -20 C for 2 h, followed by centrifugation for 10 min at 4000 rpm at 4 C

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Clin Pharmacol Ther 48(4):381389 [DOI] [PubMed] [Google Scholar] Bramness JG, Skurtveit S, Gulliksen M, Breilid H, Steen VM, Morland J (2005) The CYP2C19 genotype and the use of oral contraceptives influence the pharmacokinetics of carisoprodol in healthy human subjects