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POPs are mainly excreted through conjugation with GSH and this mechanism is extremely important for the health status, since exposure to POPs has been associated to diabetes, cardiovascular diseases and many other chronic diseases ( ROS production can occur at several intracellular sites but, for most cells, takes place in the mitochondria and the mitochondrial electron transport chain is the main cellular process of ROS generation in physiological circumstances ( 2 O 2 )
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The TgAPParc mouse model, which carries the E693G mutation (APP695) under the control of the murine Thy1.2 promoter, exhibited 3- to 7-fold higher mutant human APP expression compared to endogenous mouse APP
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For the analysis of deglutamylation activities toward -glutamyl- S -allyl-L-cysteine and -glutamyl- S -allyl-L-cysteine sulfoxide, the enzyme assay reaction mixture consisted of 0.67 g l -1 yeast crude protein, 50 mM Tris-HCl (pH 8.0), 10 mM glycylglycine, and 1 mM -glutamyl- S -allyl-L-cysteine or -glutamyl- S -allyl-L-cysteine sulfoxide was incubated for 6 h at 37C
