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For gene knockdown experiments, VSMCs were plated in 6-well dishes, cultured for 24 h in the medium without antibiotics and then transfected with shRNAs using Lipofectamine 2000 (Invitrogen Inc, Carlsbad, CA, USA) according to the manufacturer's instructions

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Malvagia, S., Papi, L., Morrone, A., Donati, M