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2.6 Assay of GSAAT activity of recombinant proteins and plant extracts In-planta GSAAT assays were performed as described previously ( 2 and 0.4% 4-NN-dimethylamino)benzaldehyde) was added, and finally absorption was recorded at 553 and 526 nm as described by In-vitro GSAAT assays were performed with 5 g of His-GSAAT, pre-incubated with either DTT or diamide, or left untreated (UT), prior to the addition of 1-3 M His-TRX f1 or His-TRX m1 for 10 min at RT