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Due to time constraints imposed by our grant and the limited availability of aged rats from the National Institute of Aging, we opted to use 25-month-old Fischer 344 rats for this study

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The cigarette, closed or lowered gaze, slumped posture, and dark doorway give it a strong feeling of exhaustion and quiet defeat

Burning smell

The e-cigarette refills used contained 50% propylene glycol (PG) and 50% vegetable glycerine (VG) and no nicotine or flavours