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Cultures were dislodged from the insert with 200 L apical dissociation solution (Accutase with 5 mM EDTA and 5 mM EGTA) for 30 min at 37 C with occasional manual agitation 68

Their findings are published in Frontiers in Immunology , the world's most-cited immunology journal

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According to The Awls annual cigarette price check, for which they call delis in each state and ask how much a pack of cigarettes costs, New York clocks in with the most expensive, at more than $14 a pack