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Briefly, each component was mixed in a final volume of 20 l with the following concentrations prior to injection of wild type animals: 50 ng/l Cas9 plasmid (pDD121, Cas9 driven by eft-3 promoter), 50 ng/l lag-1 small guide RNA (sgRNA) plasmid ( lag-1 sgRNA sequence atacagtaatcccgcgagag was cloned into plasmid DR274 U6 through BsaI site), 10 ng/l lag-1 specific repair template and 2.5 ng/l myo-2p :: gfp co-injection marker

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