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Molecular replacement was performed in Phaser 70 using the structure with PDB code 6I2G as the search model 23

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Major sources include: Foods Rich in Vitamin B12 Vitamin B12 is naturally found primarily in animal-based foods, such as: Beef liver and pork liver (the most concentrated sources) Red meat, poultry, and fish Eggs, especially egg yolks Shellfish such as clams and oysters Fatty fish like salmon, tuna, and sardines Dairy products including cows milk, yogurt, and cheese For individuals who follow a vegetarian diet, Vitamin B12-fortified foods can be a helpful choice, such as fortified cereals or fortified soy milk

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Considering the position of the Gly 1228, we could argue that the mutation G1228D will trigger a steric hindrance with T998 that is tightly constrained by F583 and cannot move easily in the apo and LTC 4 -bound conformations