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AOD-9604 is a synthetic peptide fragment derived from the C-terminal (176191) sequence of human growth hormone (HGH)

Peptides were injected onto a 75 m 2 cm trap column (Thermo Fisher) before re-focusing on an Aurora Elite 75 m 15 cm C18 column (IonOpticks) using a Vanquish Neo UHPLC (Thermo Fisher) connected to an Orbitrap Astral mass spectrometer (Thermo Fisher)

(More information) The two main classes of organosulfur compounds found in whole garlic cloves are L-cysteine sulfoxides and -glutamyl-L-cysteine peptides

-ACTIN was used as a loading control