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The fluorescence quantum yield (QY) of roGFPs was determined by comparison to Rhodamine 6G (Sigma-Aldrich, www.sigmaaldrich.com) which has a QY of 0.9 when dissolved in water (Magde et al., In vitro characterization of roGFP variants In vitro characterization of roGFP2-iL and GRX1-roGFP2-iL fusions by ratiometric time-course measurements with isolated proteins was performed on a fluorescence plate reader (POLARstar Omega